Methods of Collecting Gingival Crevicular Fluid

Gingival Crevicular Fluid

Write short note on modes of collection of sulcular fluid.
Answer. Following are the modes of collection of sulcular fluid, i.e. the use of absorbing paper strips, twisted threads placed around and into the sulcus, micropipetts, and intracrevicular washings.

• The absorbing paper strips are placed within the sulcus (intrasulcular method) or at its entrance (extrasulcular method). The placement of the filter paper strip in relation to the sulcus or pocket is important.
• Preweighed twisted threads were used. The threads were placed in the gingival crevice around the tooth, and the amount of fluid collected was estimated by weighing the sample thread.
• The use of micropipetts permits the collection of fluid by capillarity. Capillary tubes of standardized length and diameter are placed in the pocket, and their content is centrifuged and analyzed.

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• Crevicular washings can be used to study GCF from clinically normal gingiva. One method uses an appliance consisting of a hard acrylic plate covering the maxilla with soft borders and a groove following the gingival margins; it is connected to four collection tubes. The washings are obtained by rinsing the crevicular areas from one side to the other, using a peristaltic pump.
• A modification of the previous method uses two injection needles fitted one within the other such that during sampling, the inside (ejection) needle is at the bottom of the pocket, and the outside (collecting) needle is at the gingival margin. The collection needle is drained into sample tube by continuous suction.