Xylene Substitutes
“What are xylene substitutes? A detailed question and answers guide”
Question 1. Xylene.
Answer:
Properties:
- Insoluble in water.
- Soluble in aromatic hydrocarbons.
- Flammable.
Uses:
- Result
- Easily pierced Absence of precipitate
- Used as a clearing agent to remove alcohol from the tissue. To remove paraffin from dried microscopic slides.
- After staining, slides are put in xylene prior to mounting. Used to dissolve gutta percha in endodontic treatment.
- As the solvent to remove synthetic immersion oil from the microscope.
“Understanding xylene substitutes through FAQs: Composition, uses, and benefits explained”
Question 2. Alkaline phosphatase/Robinson’s Alkaline phosphatase.
Answer:
Alkaline phosphatase was first described by Robinson J.C.
- It is associated with the production of mineralized tissue.
- In hard connective tissue, it is found in the organic matrix.
- It hydrolyzes phosphate ions from organic radicals at alkaline pH.
- It cleaves pyrophosphate thereby permitting crystal growth to proceed.
- It is associated with osteogenesis and dentinogenesis.
“Importance of studying xylene substitutes for histology students: Questions explained”
- The osteoblasts and preosteoblasts exhibit alkaline phosphatase activity.
- It is seen in chondrocytes and cartilage matrix also.
- In human gingiva, the capillary endothelium of the lamina propria shows its activity.
- Alkaline phosphatase is involved in the mechanism of keratinization.
- Basement membrane associated with salivary gland acini and taste buds exhibits high alkaline phosphatase activity.
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