Soft Tissue Processing

Soft Tissue Processing

“How do adipocytes contribute to tissue composition? FAQ explained”

Question 1. Stages in soft tissue processing.
Answer:

Stages:

• Obtaining the specimen.
• Fixation Ideally with 10% formalin.
• Dehydration with increasing strength of alcohol.
• Clearing.
• By passing through xylene
• Infiltration by paraffin.
• Embedding the specimen in paraffin wax block.
• Cutting to obtain desired sections of tissues
• Mounting the sections on slides.
• Staining with the desired staining method.

“Understanding soft tissue processing through FAQs: Composition, functions, and uses explained”

Soft Tissue Processing

Question 2. Declassification.
Answer:

It is a technique for removing minerals from bone and other calcified tissue so that good paraffin sections can be prepared.

Chemical used:

• 5% nitric acid.

Time required:

• 7-10 days for complete decalcification.

Tests:

“Importance of studying soft tissue processing for pathology students: Questions explained”