Irreversible Steps Of Glycolysis

Irreversible Steps Of Glycolysis

Gluconeogenesis/Describe Irreversible steps of glycolysis.
Answer:

Definition:

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The synthesis of glucose from non-carbohydrate compounds is known as gluconeogenesis.

Location:

1. It is located in the cytosol
2. Mostly occurs in the liver

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Reactions:

• Closely related to the reversed pathway of glycolysis.
• Besides, it overcomes the irreversible steps of glycolysis,
• The irreversible steps of glycolysis are:

Thermodynamics of glycolysis irreversible steps

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Conversion of pyruvate to phosphoenol private.

1. It occurs in two steps.
2. Pyruvate converted to oxaloacetate
   • It occurs in mitochondrial by the enzyme pyruvate carboxylase in the presence of ATP and CO2.
   • As oxaloacetate cannot diffuse out of the mitochondria, it is converted to malate by malate dehydrogenase and transported to cytosol.
3. Oxaloacetate is converted to phosphoenol pyruvate.

It occurs in the mitochondrial matrix as well s cytosol by the enzyme phosphoenolpyruvate carboxykinase.

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2. Conversion of fructose 1; 6 – bisphosphate to fructose 6 – phosphate.

• Fructose 1,6- bisphosphatase converts fructose 1, 6 – bisphosphate to fructose 6 – phosphate.

3. Conversion of glucose 6 – phosphate to glucose.

• It is catalyzed by glucose 6 – phosphatase.