Fructose Metabolism

Fructose Metabolism

Detection of fructose in urine.
Answer:

Fructose is a reducing sugar and ketohexoses.

“Factors influencing success with fructose metabolism studies: Q&A”

1. Test for reducing property:

1. Benedict’s test:
   • Fructose form enediols
   • The enediol forms of sugars reduce cupric form to a yellow precipitate of cuprous hydroxide or a red precipitate of cuprous oxide.
2. Barfoed’s test:

“Understanding fructose metabolism through FAQs: Pathways, functions, and uses explained”

Similar to Benedict’s test but carried out in a slightly acidic medium.

2. Test for detection of fructose as ketohexoses.

1. Seliwanoffs test:
   • Concentrated HCI dehydrates ketohexoses to form furfural derivatives.
   • This condenses with resorcinol to give a cherry red complex.

“Importance of studying fructose metabolism for medical students: Questions explained”

1. Toulge/s test:
   • The furfural derivatives of ketohexoses condense with urea in the presence of stannous chloride to give blue color.
2. Rapid furfural test:
   • Ketohexoses are converted to furfural derivatives by 11C1.
   • This forms a purple color complex with α-naphthol.