Bile Pigments And Bile Salts: Formation, Function, And Lab Tests

Bile Pigments And Bile Salts: Formation, Function, And Lab Tests

Question 1. Bile pigment formation
Answer:

• Bile pigments are biliverdin & bilirubin
• They are formed by heme degradation

Question 2. What are bile salts? How are they tested in the laboratory?
Answer:

• Bile salts are the sodium & potassium salts of bile acids which are conjugated with glycine or taurine

Tests to detect bile salts:

1. Hay’s test

• In this test, the sulfur powder is sprinkled on the surface of urine containing bile salts
• As a result, bile salts sink to the bottom

2. Petternkofer’s test

• Sugar is reacted with concentrated sulphuric acid
• The furfural derivatives condense with bile salts to form a purple ring

Question 3. Define pH. Mention the normal range of blood pH.
Answer:

Definition:

• It is a numeric scale used to specify the acidity or alkalinity of an aqueous solution On this scale
• 7-neutral
• A lower value indicates acidic
• A higher value indicates alkalinity pH stands for the potential of Hydrogen

Normal range of blood pH

• The normal range of blood pH is 7.35 -7.45