BANA Test

BANA Test

Write short note on BANA Test.
Answer. BANA Test (N-benzoyl-DL-arginine-naphthylamide)

• BANA test is highly sensitive, inexpensive and is easy to use chair side test for periodontal risk.
• In just 5 minutes BANA test can detect bacteria associated with periodontal disease simply by applying tongue swabbing or sub – gingival plaques to a small test strip.
• BANA test is the modifiation of BANA hydrolysis test which is developed by Dr. Walter Loesche and colleagues at University of Michigan School of Dentistry.

“Importance Of Preventing Bad Breath For Oral Health”

• It exploits an unusual tyrosine like enzyme which is found in Treponema denticola, porphyromonas gingivalis and bacteroides forsythus, three anaerobic bacteria which are highly associated with adult periodontitis.
• Of 60 subgingival plaque species, only these three possess an enzyme capable of hydrolyzing the synthetic trypsin substrate, benzyol-DL-arginine-naphthylamide (BANA) present on the BANA test strips.
• If any of three species is present, they hydrolyze BANA enzyme producing B-naphthylamide which in turn reacts with embedded diazo dye to produce a permanent blue color which indicate a positive test.

“Understanding The Role Of Bacteria In Causing Halitosis”

“Risk Factors For Developing Chronic Halitosis”

How the Test Works

For periodontal risk assessment, subgingival plaque is obtained with a curett. Samples are placed on BANA test strip, which is then inserted into the slot on a small toaster-sized incubator. The incubator automatically heats the sample to 55°C for 5 minutes.

“Early Signs Of Halitosis And How To Detect It”

If P. gingivalis, B. forsythus or T. denticola are present, test strip turns blue. The blue it turns, higher is the concentration and the greater is the number of organisms.