Direct vs. Indirect Immunofluorescence: Diagnostic Techniques Explained

Direct vs. Indirect Immunofluorescence: Diagnostic Techniques Explained

Question. Write short note on direct and indirect immunofluorescence.

Answer.

“Role of antibodies in direct immunofluorescence: Questions answered”

Direct and indirect immunofluorescence are the techniques that are used to detect immune-mediated or immunobullous diseases.

direct vs indirect immunofluorescence

Direct immunofluorescence

It is the diagnostic method whiacch is used for detection of autoantibodies which are bound to patient’s tissue.

“Understanding the role of immunofluorescence in diagnostic pathology: Q&A explained”

Method

• Frozen section of patient’s tissue is placed over a slide.
• Tissue is incubated with fluorescein­conjugated goat antihuman antibodies.
• Antibodies bind to the human immunoglobulin site.
• Excess of antibodies are washed with buffred normal saline.
• Section is viewed under ultraviolet microscope.

“Importance of studying immunofluorescence techniques for better outcomes: Questions explained”

Indirect Immunofluorescence

It is the diagnostic method that is used for the detection of antibodies which are present in blood.

indirect immunofluorescence test

Method

• Frozen section of monkey’s esophagus is placed over a slide and is incubated with patient’s serum.
• Excess serum is washed off
• Apply fluorescent conjugated anti-human Ig antibodies.
• Wash of excess antibody.
• Section is viewed under ultraviolet microscope.

“Common challenges in using direct vs indirect immunofluorescence effectively: FAQs provided”

“Steps to explain applications of direct vs indirect immunofluorescence: Autoimmune diseases vs infections: Q&A guide”