Alkaline Phosphatase

Alkaline Phosphatase

Question 1. Discuss the clinical importance of alkaline phosphatase.
Answer:

• Alkaline phosphatase is increased in
• Rickets
• Hyperparathyroidism
• Bone disorders
• Obstructive jaundice
• It’s alpha 2 heat labile isoenzyme increases in hepatitis
• Its pre-beta isoenzyme indicates bone diseases

Question 2. Discuss the clinical importance of acid phosphatase with its normal range.
Answer:

Acid Phosphatase Normal range:

• 0-2.5 units King Armstrong

Acid Phosphatase Importance:

• Increased in prostatic carcinoma

Question 3. What is the Km value in enzyme reaction?
Answer:

• Km value is defined as the substrate concentration to produce half maximum velocity in an enzyme-catalyzed reaction
• Km value is constant and a characteristic feature of a given enzyme
• It is representing the strength of the enzyme-substrate complex
• A low Km value indicates a strong affinity between enzyme and substrate
• A high Km value indicates a weak affinity between them

Question 4. Suicide inhibition
Answer:

• Suicide inhibition is a type of irreversible inhibition
• In this, the original inhibitor is converted to a more potent form by the same enzyme that ought to be inhibited

Suicide inhibition Example:

• Allopurinol, an inhibitor of xanthine oxidase gets converted to alloxanthine a more effective inhibitor of the enzyme

Suicide inhibition Significance:

• Used in cancer therapy.