Protein Electrophoresis

Protein Electrophoresis

Explain the separation of plasma proteins by electrophoresis.
Answer:

“Factors influencing success with protein electrophoresis studies: Q&A”

• Electrophoresis is the most commonly employed analytical technique for the separation of plasma proteins
• The movement of charged particles in an electric field resulting in their migration towards the oppositely charged electrodes is called Electrophoresis

It depends on:

• Shape, size, net charge & solvation of the ions
• The viscosity of the solution
• The magnitude of the current employed

“Understanding protein electrophoresis through FAQs: Composition, functions, and uses explained”

Method:

1. Paper or gel Electrophoresis is employed for the separation of plasma proteins
   1. Paper Electrophoresis
      • In this, first, the sample is applied on a strip of Whatman no. 1 filter paper with wetted with veronal buffer
      • The ends of the strip are dipped into the buffer reservoirs in which the electrodes are placed
      • Electric current is applied
      • The paper is removed, dried & stained with amido black or bromophenol blue
      • As a result, the plasma proteins are separated into five distinct bands namely albumin, alpha 1, alpha 2, beta & gamma globulins
      • The concentration of each can be estimated by a densitometer Gel Electrophoresis
   2. It involves the separation of molecules based on their size
      • Thus the movement of large molecules is slow in gel
      • Plasma proteins are separated into about 15 bands
      • Densitometer scanning

“Importance of studying protein electrophoresis for biochemistry students: Questions explained”